DNA Testing Options 

DNA Testing Options 

Genomic SNP 

The Genomic SNP (Single Nucleotide Polymorphism) test analyses a high-density panel of genetic markers within an animal’s DNA profile. This information is used to verify dam and sire parentage both individually and, in combination with the calf (as a trio), to confirm that parentage is correctly assigned to the calf’s DNA profile. 

The Australian Wagyu Association (AWA) currently works with three approved laboratories: Zoetis (approximately 50,000 SNP markers), Weatherby’s (approximately 85,000 SNP markers), and Neogen (approximately 100,000 SNP markers). This test is the foundation for animal registration and is required before requesting additional genomic services such as the Wagyu Content Check test. 

Wagyu Content Check (WCC) 

The Wagyu Content Check (WCC) uses genomic analysis derived from the SNP profile to estimate the proportion of Japanese Black Wagyu genetics in animals that do not have a fully traceable pedigree. The analysis compares the animal’s genotype against the Wagyu reference population to determine its genetic similarity. 

This test is typically requested after or at the same time a genomic SNP result has been processed and is commonly used where parentage is unknown or where animals are the result of crossbreeding. The result determines the animal’s grade classification within the AWA database. 

Table 1: Grade Code Assignment Based on Generation or Minimum % Wagyu Content 

Table 1: Grade Code Assignment Based on Generation or Minimum % Wagyu Content 

GRADE
GENERATION
MIN. % WAGYU CONTENT
GRADE CODE IN IDENT
0
0 = Base Animal 
< 50% 
0 
1
1 = Crossbred Wagyu F1
50% – <75%  
1 
2
2 = Crossbred Wagyu F2 
75% – <87.5%  
2 
3
3 = Crossbred Wagyu F3 
87.5% – <93.75%  
3 
4
4 = Crossbred Wagyu F4 
>93.75%  
4 
PUREBRED
Purebred 
>93.75% + and Parent Verified to sire and dam. 
P 

FULLBLOOD
Fullblood 
Parent Verified to fullblood registered dam and sire.  
F 
 

Visit Wagyu Content Check , for further information. 

Wagyu Feeder Check 

Wagyu Feeder Check is a commercial genomic test designed for Wagyu-content cattle to support decision-making in long-fed programs. It identifies animals with lower genetic merit, helping producers improve overall program performance. 

The test provides genomic predictions for key production traits such as growth, carcase weight, fat depth, eye muscle area and marbling, and also includes sire verification. It is most commonly used for first and second crossbred animals and is available exclusively through Neogen. 

Testing must be conducted using TSU samples, and a new sample must be collected, as existing samples at the laboratory cannot be reused for this test.  

Additional Testing 

Additional DNA testing options are available to provide further insight into genetic conditions and phenotypic traits. These tests can be requested as part of bundle packages or ordered separately, depending on your requirements. 

Genetic condition testing includes screening for known conditions such as B3, CHS, CL16, F11, F13, IARS and ANXN10. These tests assist in identifying carriers and support informed herd health and breeding decisions. 

Trait testing includes genetic tests for characteristics such as POLL (horn status), coat colour, dilution factor, tenderness and SCD, which influence how animals perform and the quality of their carcase. 

Bovine Viral Diarrhoea Virus (BVDV) testing can only be performed using a TSU sample and must be submitted in conjunction with a genomic SNP test. Standalone BVDV testing is not available through AWA laboratory services.  

Testing services are offered through Neogen, Weatherby’s and Zoetis as part of bundled packages. Pricing for all additional testing options is available in the AWA Fee Schedule.

 Visit Genetic Conditions & Traits, for further information. 

RELATED

When submitting a DNA test request, consideration should be given to the choice of laboratory, sample type and the intended outcome of the test.
The Genomic SNP (Single Nucleotide Polymorphism) test analyses a high-density panel of genetic markers within an animal’s DNA profile.

DNA parentage analysis within the Australian Wagyu Association (AWA) is conducted in two stages: parent verification and, where required, parent discovery.
DNA testing is a mandatory requirement for registering animals and accessing Wagyu Breeding Values (WBVs) within the database.

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IMPORTANT NOTICE - Terms & Conditions


It is a condition of use of this database that you accept these terms and conditions. 

It is important that you appreciate when accessing the Australian Wagyu Association Limited (AWA) database (which may be hosted on an external website) that the information contained on the AWA database, including but not limited to pedigree, DNA information, Wagyu Breeding Values (WBVs) and Index values (together called Database Information), is based on data supplied by AWA members and/or third parties. 

Whilst sincere effort is made to ensure the Database Information is accurate and complete, to the extent permitted by law, AWA, its officers and employees, shall not be responsible for its use or interpretation, and you use the Database Information at your own risk. Please be aware of the following limitations of the Database Information.  

Regarding WBVs and Index values, it is important to appreciate, and you need to be aware that: 

  • WBVs are derived using genetic evaluation technology developed for the AWA, using information contained within the AWA database and parameters derived from it.
  • WBVs are estimates of genetic potential of individual animals and may change with addition of new data or with new parameters.
  • WBVs are genetic merit estimates only and may not reflect the raw animal phenotype.
  • WBVs can only be directly compared to other WBVs calculated in the same AWA genetic analysis.

Regarding pedigree and DNA testing results submitted to the AWA, it is important to appreciate, and you need to be aware that: 

  • Animals whose registration identifiers start with PED are Pedigree Recorded Animals. What this means is that the details which AWA used to register the animal were obtained from a Recognised Wagyu Registry Organisation – in other words a Wagyu breed association based outside of Australia. AWA has not independently verified the information with respect to the animal or the details recorded about the animal. You should read AWA’s by-laws which set out details regarding the definition of a Pedigree Recorded Animal. 
  • Pedigree and DNA data submitted and supplied to AWA may have errors in it which cannot be detected without further DNA testing. 
  • Technology may have advanced since a particular test was undertaken so that genetic issues or inaccuracies which were previously not detectable are now able to be detected by current testing technology (i.e. if the animal was tested again with current technology). 
  • AWA estimates that less than 1% of the pedigree entries, ownership or breeding details in the AWA Herdbook may have errors or which may be misleading. For this reason, users ought to consider if they need to obtain independent testing of the relevant animal (if possible) to ensure that the data is accurate. 

Regarding prefectural content, it is important to appreciate, and you need to be aware that: 

  • Prefectural content is based on the estimation of prefectural origin from Japanese breeding records of 201 foundation sires and 168 foundation dams. As genotype-based parent verification is not used in Japan, and full Japanese registration certificates are not available for all foundation animals, exact prefectural composition for these sires and dams cannot be validated. 
  • The calculation of prefectural content for Australian Herdbook animals relies on the accuracy of pedigree records and DNA samples provided by AWA members.   

Regarding DNA testing and genetic condition results submitted to the AWA, it is important to appreciate, and you need to be aware that: 

  • As genetic testing results are provided by independent DNA testing laboratories at the request of AWA members, AWA relies solely on the accuracy of the reporting of genetic testing as completed by the laboratories. 
  • AWA uses GeneProb software to calculate the likelihood of pedigree animals being a carrier of genetic conditions.  This likelihood is an estimate based on the available genetic testing results. 

If you consider that you do not understand or appreciate the nature and/or implications of the data provided on this website or the WBVs of a particular animal, then AWA strongly recommends that you seek expert advice.  Note there is substantial technical information and articles on the Wagyu website to assist our members. 

AWA’s liability for any loss or damage, consequential or otherwise, suffered or incurred by you or your related business, arising directly or indirectly from your use of Database Information, to the extent permitted by law, is limited to AWA providing you with a refund for the fees paid for the service (if any), or AWA re-performing the service, where possible, at the election of AWA.  

AWA administers this database in good faith to support AWA members and the industry. AWA does not represent or warrant that the animals recorded in this database, or animals breed from them, will achieve any particular genetic or phenotype performance.


THIS DISCLAIMER HAS BEEN UPDATED AS OF 03/02/2026, PLEASE ENSURE YOU HAVE READ IT IN DETAIL